Trehalose-6-phosphate synthetase activity in extracts of Dictyostelium discoideum☆

[摘要] Trehalose-6-phosphate(T-6-P)synthetaseactivityinextractsofDictyosteliumdiscoideumhasbeenreexaminedinanefforttoresolvediscrepanciesbetweentheresultsofpreviousstudies(R.RothandM.Sussman(1966).Biochim.Biophys.Acta,122,225;K.A.KillickandB.E.Wright(1972).J.Biol.Chem.,247,2967).WefindthatT-6-PsynthetaseisnotcoldsensitiveasreportedbyKillickandWright(1972),isnotpresentinbacterial-grownvegetativecells(thoughsubjecttosomemodulationbyothernutritionalconditions),andisnotinourhandsunmaskedoractivatedbyammoniumsulfatefractionation.WeconcludethatthepatternofT-6-PsynthetaseaccumulationanddisappearanceduringfruitingbodyconstructioninD.discoideumisasoriginallydescribedbyR.RothandM.Sussman(1968).J.Biol.Chem.,243,5081)andconfirmedelsewhere(P.C.Newelletal.(1972).J.Mol.Biol.,63,373;R.W.Brackenburyetal.(1974).J.Mol.Biol.,90,529;B.D.HamesandJ.M.Ashworth(1974).Biochem.J.,142,301).

[学科分类] 生物科学（综合）