Appearance of DNA polymerase activities during early development of Xenopus laevis
[摘要] ExtractsoflargeoocytesofXenopuslaeviscontainhighlevelsofonemajorDNApolymeraseactivity.Aftermaturationintoeggs,theoveralllevelofDNApolymeraseactivityinextractsincreasesfourfoldandasecondmajoractivityappearsonSephadexG-200orDEAEcellulosecolumns.AlthoughintenseDNAsynthesisoccursasthenumberofcellsincreasefromonetoover100,000,nofurtherincreasesinthelevelofeitherDNApolymeraseactivityareobservedincleavage,gastrulaorearlyneurulastageembryos.Inextractsoflateneurulaeorhatchedembryos,however,athirdmajorDNApolymeraseactivityappearscoincidentwithanincreaseintheabilityoftheextractstoutilisenativeDNAtemplatesinvitro.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学(综合)
[关键词] [时效性]