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Appearance of DNA polymerase activities during early development of Xenopus laevis
[摘要] ExtractsoflargeoocytesofXenopuslaeviscontainhighlevelsofonemajorDNApolymeraseactivity.Aftermaturationintoeggs,theoveralllevelofDNApolymeraseactivityinextractsincreasesfourfoldandasecondmajoractivityappearsonSephadexG-200orDEAEcellulosecolumns.AlthoughintenseDNAsynthesisoccursasthenumberofcellsincreasefromonetoover100,000,nofurtherincreasesinthelevelofeitherDNApolymeraseactivityareobservedincleavage,gastrulaorearlyneurulastageembryos.Inextractsoflateneurulaeorhatchedembryos,however,athirdmajorDNApolymeraseactivityappearscoincidentwithanincreaseintheabilityoftheextractstoutilisenativeDNAtemplatesinvitro.
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[效力级别]  [学科分类] 生物科学(综合)
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