Permeability of Xenopus laevis embryos: Specific incorporation of precursors into eukaryotic proteins and nucleic acids☆☆☆
[摘要] AllaminoacidsandseveralnucleicacidprecursorsaretakenupbyXenopuslaevisembryos.Theembryosarecompletelyintactandnotmodifiedinanyway.TheseprecursorsaredirectlyincorporatedintothemacromoleculesofXenopusembryosandnotprokaryoticcontaminantsashasbeenpreviouslyclaimed.RadioactiveleucineisincorporatedintoXenopuslaevisribosomalproteinsascharacterizedbysucrosegradientcentrifugation.Theuptakeoftheaminoacidsiscycloheximidesensitiveandunaffectedbychloramphenicol.RadioactiveadenosineandoroticacidaretakenupandincorporatedintotRNAandrRNAathighlevelsascharacterizedbysucrosegradientsandelectrophoresis.ThesecharacterizationsoflabeledmacromoleculesunequivocallyshowthatnormalXenopuslaevisembryoswilltakeupandincorporatelabeledprecursorstolevelswhicharesufficienttostudycellularbiochemicaleventsatsuchearlystagesofdevelopment.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
