An autoradiographic study of the origin of intestinal blastemal cells in the newt, Notophthalmus viridescens☆

[摘要] Fiftyadultnewtswereusedinthisinvestigation;in44animals,theintestinewastransectedperpendiculartoitslongitudinalaxisapproximatelymidwaybetweenpylorusandrectum.Thefreeendsoftheintestinewereheldinappositionwithasinglesutureandreplacedintothecoelom.Theanimalswereinjectedintraperitoneallywith[3H]thymidinefrom0to35daysaftertransectionoftheintestineandkilled6hrlater.Innontransected,controlintestines,theonlytissuethatincorporated[3H]thymidinewasthemucosalepithelium.Intransectedintestines,onlythemucosalepitheliumlabeledinanimalswhichhadbeeninjectedwith[3H]thymidinefrom0to4daysaftertheintestinewasincised.Lateron,serosalcellsandsmoothmusclecellsoftheintestinalstumpunderwentmorphologicalalteration,initiatedtheincorporationof[3H]thymidineintoDNA,andbeganreplication.At6daysaftertransection,serosalcellsadjacenttotheplaneoftransectionwereincorporating[3H]thymidineand,at12days,smoothmusclecellsatthetransectedsurfacewerelabeling.Itseemsprobablethattheybothfurnishedcellstotheintestinalblastema;theliningepitheliumofthemucosa,however,didnotappeartocontributetotheblastemaproper.

[学科分类] 生物科学（综合）