Fatty acid synthesis in chick embryonic heart and liver during development☆
[摘要] Fattyacidsynthesisbysubcellularfractionsofheartandliverofchickembryosatvaryingstagesofdevelopmenthasbeenstudied.Fattyacidsynthetaseactivityisassociatedwiththeembryonicheartatearlystagesofdevelopment,assuggestedbysubstraterequirement,Schmidtdecarboxylationofsynthesizedfattyacidsandgasliquidchromatographicidentificationoftheproductsaspalmiticandstearicacids.Thefattyacidsynthetaseactivitydecreasesinheartcytosolwithageoftheembryoandisabsentinthenewlyhatchedchickandinolderchicken.TheacetylCoAcarboxylaseactivityisnegligibleinembryonicandadultchickenheart.Thefattyacidsynthetaseactivityinliverislow,butmeasurableduringtheentireembryonicdevelopment.Theactivityincreasesbyaboutthree-foldonhatchingandthereafterinfed,newlyhatchedchicksbyabout35-fold,overthebasalembryonicactivity.Theacetylandmalonyltransacylaseactivitiesintheheartandlivercytosolsduringdevelopmentfollowedcloselythefattyacidsynthetaseactivitiesinheartandliver,respectively.Anon-coordinateinductionoffattyacidsynthetaseandacetylCoAcarboxylaseactivitiesinliverwasobservedduringdevelopment.Themicrosomalchainelongationinliverandheartfollowedthepatternoffattyacidsynthetaseactivityinliverandheart,respectively.Themitochondrialchainelongationinembryonicheartisinitiallylowandincreaseswithage;whilethisactivityinliverishigherinearlystagesofembryonicdevelopmentthanintheolderembryosandthechicks.Measurementoflipogenesisfromacetate-1-14Cbyliverandheartslicesfromchickembryosandnewlyhatchedchickssupporttheconclusionsreachedinthestudieswiththesubcellularfractions.Theresultsobtainedindicatethatthemajorsystemoffattyacidsynthesisinembryonicandadultheartisthemitochondrialchainelongation.Inembryonicliver,fattyacidsynthesisproceedsbychainelongation,whilethedenovosystemisthemajorcontributortothelipogeniccapacityoftheliverafterhatching.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
