Long term organ culture of rat liver rudiment in a synthetic medium: Morphological and biochemical development☆
[摘要] Embryonicratliverrudimentsweregrownandmaintainedinachemicallydefinedmediumforupto16weeks.Liverexplantsgrewanddevelopedliverplates,bilecanaliculiandductules.Livercellsdifferentiatedand,bytheendofthesecondcultureweek,revealedcytologicalandultrastructuralfeaturesofadultratlivercells.Glycogensynthetaseactivityappearedandreachedaplateauwithin2weeks.Plasmaproteinsproducedbytheexplantsappearedsequentiallyintheculturemedium.Theirrelativeconcentrationsinthemediumincreasedprogressivelytoaplateauandtheypersistedthroughoutthecultureperiod.Albuminwaspresentintraceamountsduringearlydaysofculture.Itsrelativeconcentrationincreasedanditbecamethemajorproteinconstituentofthemediumbythesecondweek.Transferrin,α1M-globulin,haptoglobin,β1c-andα1B-globulinappearedinthatorder.Thethreefibrinogensubunitswereidentifiedduringthefirstweekofculture.Fetalproteinswereidentifiedduringtheearlycultureperiod,themostprominentbeingα-fetoprotein.Therelativeconcentrationoffetalproteinsdeclinedsteadilyasthecultureprogressed.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
