Contribution of a change in mRNA half-life to the accumulation of the tissue-specific S-100 protein during postnatal development of the mouse brain☆
[摘要] TheS-100proteinaccumulatesrapidlyinthemousebrainbetween15and21daysofpostnataldevelopment.Theaccumulationofthisproteinisbroughtaboutmainlybyanincreasedrateofitssynthesis.Thepresentstudyfocusesonattemptstodetermineifachangeinthehalf-lifeofmessengerRNA(mRNA)isinvolvedinbringingabouttheincreasedrateofsynthesisoftheS-100protein.UtilizingtheinhibitionofRNAsynthesisbyactinomycinD,wewereabletoshowthatthehalflifeofmRNAincreasesconcurrentlywithanincreaseintherateofsynthesisoftheS-100protein.UtilizationofactinomycinDdoespresenthazardsininterpretationofresultsonmRNAstability;experimentswereperformedtodetermineiftheresultsobtainedwereduetosideeffectsofthedrug.Asfarascouldbedetermined,thepossiblesideeffectsofactinomycinDdidnotaffectourresults.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
