Different mechanisms for the induction of acetylcholinesterase in neuroblastoma cells
[摘要] Neuroblastomacells,inclidingclonesselectedforresistancetodibutyryl-cAMPorfortheirabilitytosurviveandmultiplyat40°C,wereusedtostudydifferencesintheinductionofacetylcholinesteraseactivitybydibutyryl-cAMPand5-bromodeoxyuridine.Innonselectedneuroblastomacellsbothofthesecompoundsinducedthisenzymeactivity.ActinomycinDinhibitedinductionby5-bromodeoxyuridinebutdidnotinhibitinducionbydibutyryl-cAMP.Enzymeactivityindibutyryl-cAMP-resistantcellswasinducedby5-bromodeoxyuridineandnotbydibutyryl-cAMP.Inthetemperature-resistantcells,inductionby5-bromodeoxyuridinewaslowerat40thanat37°Candinductionbydibutyryl-cAMPwashigherat40thanat37°C.Thisdifferenceininductionatthetwotemperatureswasassociatedwithahigherinhibitionofcellmultiplicationat40°Cbybothcompounds.Theresultsindicatethat5-bromodeoxyuridineanddibutyryl-cAMPinduceacetylcholinesteraseactivityinneuroblastomacellsbydifferentmechanisms.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
