Structural and functional changes of myosin during development: Comparison with adult fast, slow and cardiac myosin☆
[摘要] ATPase(Ca2+andK+activated)activityofmyosinpreparedfrommusclesof3–4weekrabbitembryos(EM)isslighlylowerthanthatofadultfastmusclemyosin(FM),butincontrasttothelessactiveadultslowmusclemyosin(SM)isstableonexposuretopH9.2.Studiesofthetimecourse,bymeansofNadodecyl-SO4polyacrylamidegelelectrophoresis,ofchangesinthepatternofpolypeptidesreleasedbytrypticdigestionshowthatinthisregardEMisclosesttoSM.ThelightchaincomplementofEMappearsidenticalwiththatofFMratherthanofSMorcardiacmyosin(CM)bythecriteriaofcoelectrophoresisandremovalby5,5′-dithio-2-dinitrobenzoatetreatmentofLC2exceptthattherelativeamountofLC3islessinEMthaninFM.Thestainingpatternoflightmeromyosin(EMM)paracrystalspreparedfromEMisdistinctfromeithertheFM,SMorCMLMMstainingpattern.Thesestudiessuggestthatdifferentgenesareinvolvedinthecodingforembryonicandadultheavychains.
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[效力级别] [学科分类] 生物科学(综合)
[关键词] LC1;light chain 1;LC2;light chain 2;LC3;light chain 3;HMM;heavy meromyosin;LMM;light meromyosin;EM;embryonic muscle;SM;slow myosin;FM;fast myosin;CM;cardiac myosin;SDS;sodium dodecyl sulfate;DTNB;5;5′-dithiobis-(2-nitrobenzoic acid);EDTA;ethylenediamine tetraacetic acid;DTT;dithiothreitol;SPAGE;SDS polyacrylamide gel electrophoresis [时效性]