A biological nuclear marker in cell culture: Recognition of nuclei in single cells and in heterokaryons☆
[摘要] Thebiologicalmarkingtechniquebasedonquailandchickcellcombinations,previouslydevisedforinvivoexperimentsandorganotypiccultures,canalsobeappliedtocellcultures.Intheexperimentsreportedinthisnote,quailandchickfibroblastsandcardiaccellshavebeencultivatedfrom2–8days.Uptotheendoftheseconddayofcultivation,thenucleolarstructureofquailcellsremainssimilartothatobservedinorganizedtissues.Afterwards,thenucleolarenlargementwhichoccursinrapidlygrowingmonolayerculturesresultsinthefragmentationofthecharacteristicnucleolus-associatedheterochromatininquailnuclei.However,sincechicknucleishowonlysmalldispersedchromocenters,cellsofthetwospeciescanbeidentifiedevenafterseveraldaysinculture.Acridineorangestainingisshowntobeaneasyandreliableprocedureforidentifyingthenuclearmarker,eveninheterokaryons.Cytoplasmstainstodistinguishcelltypescanalsobeappliedtothesamepreparations.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
