Maturation of rabbit reticulocytes: Degradation of specific reticulocyte proteins☆
[摘要] Asanalyzedbypolyacrylamide-gelelectrophoresis,rabbitreticulocytecytoplasmcontains,inadditiontoglobin,sevenpredominantpolypeptides.Theamountsoftheserangefrom0.1to1.2%ofglobin.Rabbiterythrocytescontainonlythreeofthesenonglobinpolypeptides.Thelossofthefourotherpolypeptidesiscorrelatedwithmaturationofthereticulocytestoerythrocytes.Wealsofractionatedreticulocytesaccordingtoagebyequilibriumcentrifugationthroughagradientofmetrizamideandshowedthatyoungerreticulocytescontainmuchmoreofthesefourpolypeptidesthandomorematurereticulocytes.Thefourpolypeptidesthatarelostduringdifferentiationarealsoverysensitivetoinvitrodestructionbychymotrypsinortrypsinunderconditionswhereglobinandthethreereticulocytenonglobinpeptidesthatremainduringreticulocytematurationarecompletelyresistant.Ourresultsareconsistentwiththenotionthatthedegradativerateofareticulocytecytoplasmicproteinisdeterminedbyitssusceptibilitytogeneralintracellularproteases.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
