In vitro synthesis and processing of ribosomal RNA in the housefly ovary☆
[摘要] Muscadomesticaovarieslabeledinvitrowith3H-uridinetranscribeandprocessribosomalRNA.ThemolecularweightsofrRNAprecursors,intermediatesandproductsobservedare:38S(2.85×106),32S(1.85×106)30S(1.66×106),28S(1.42×106),20S(0.76×106)and18S(0.70×106).IncubationofprelabeledovarieswithactinomycinDinhibitstranscriptionandallowscompleteprocessingof38Sprecursorthroughintermediatesinto28Sand18SRNAin40min.The38SRNAiscleavedintoa32Sand20SRNA.The32Sintermediateisfurtherprocessedtoa30Sprecursorofmature28SrRNAandthe20SRNAservesasaprecursorto18SRNA.Thehalf-lifeofthe38Sprecursorhasbeencalculatedas12min.Approximately26%ofprecursormolecularweightislostduringprocessingtomaturerRNA.Theresultsobtaineddemonstratetheproductionofinsect28SRNAthrougha32SRNAintermediateusinganinvitrosystemwhichallowscompleteprocessingofrRNA.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
