Immunological studies on the Purkinje cells from rat and mouse cerebella: I. Evidence for antibodies characteristic of the Purkinje cells☆

[摘要] AntibodieshavebeenraisedagainstanenrichedpreparationofisolatedratcerebellarPurkinjecells.Theimmunoglobulinswerelabeledwith125Iandthestrengthandspecificityoftheserumdeterminedbyadirectbindingassayoncerebellarmembranes.About2%ofthe125I-labeledIgGboundtoanexcessofcerebellarmembranes.Absorptionwithheartandlivermembranesremoved80.5%ofthe125I-labeledIgGbindingtocerebellarmembranes;absorptionwithcerebrummembranesremoved13%more;theremaining6.5%weredirectedspecificallyagainstcerebellarmembranes.Anenriched125I-labeledanti-Purkinjeantibodypopulationwaspreparedbyabsorptionandsubsequentelutionfromcerebellarmembranes.Theabsorptionpatternwithheart,liver,andcerebrummembranesresembledthatfoundwiththetotalpopulationofIgGexceptthatthenonspecificbindingwassignificantlydiminished.ThePurkinjecelldegeneration(pcd)mousemutantwasusedtoassessthespecificityoftheserumtowardthePurkinjecells.Afterabsorptionoftheenrichedanti-Purkinjeantibodieswithheart,liver,andcerebrummembranes,thebindingoflabeledIgGtomembranespreparedfrompcd/pcdcerebellawasaboutone-fourththatfoundwithcontrolcerebella.ThedirectimmunoperoxidasetechniqueperformedonsmearsofpurifiedPurkinjeandgranulecellsshowsthattheunabsorbedserumstainsbothclassesofcells,butthatafterabsorptionwithliver,heart,andcerebrummembranes,onlythePurkinjecellsreactpositively.

[学科分类] 生物科学（综合）