Immunological study of lung development in the mouse embryo: II. First appearance of the great alveolar cell, as shown by immunofluorescence microscopy

[摘要] Anantiserumthatspecificallyrecognizesalung-specificantigenpresentinthegreatalveolarcellintheadultmouselungwasusedinimmunofluorescencestudiestodetectthefirstappearanceofthisantigenintheembryo.CellularfluorescencewasfoundtooccurinthelungtissuefromaboutDay14.2onwardandtobeduetothepresenceofthelung-specificorarelatedantigen.Thesimultaneousappearanceofthisantigen(ca.Day14.2)andthecuboidaltypeofepithelialcellinwhichitoccurs(ca.Day14)meansthatthegreatalveolarcell—oritsprecursor—isfirstdetectablearoundDay14.2.Sincethegreatalveolarcell—oritsprecursor—isthefirstandonlytypeofalveolarepithelialcelltooccurintheembryoniclung,itmustbethestemcellfromwhichthesmallalveolarcellderives.Thepersistentsharpdemarcationbetweentheprospectivealveolarandbronchialepitheliaindicatesthattherespiratoryandtheconductingportionsofthelungoriginatefromdifferentpartsofthetubularsystemintheprenatallung.

[学科分类] 生物科学（综合）