Localized ultraviolet laser microbeam irradiation of early Drosophila embryos: Fate maps based on location and frequency of adult defects☆

[摘要] Drosophilaembryoswerelocallyirradiatedwitha257-nmlasermicrobeamduringblastodermandgermbandstages.Dependingonstageandbeamdiameter(10–30μm),from0to45nucleiwereexposedtotheuvradiation.Thedosesused,5or10erg,didnoteliminatenucleiorcellsatonce,butupto50%oftheadultsurvivorsfromirradiatedeggscarrieddefectsinthethorax.Thesewerescoredwithreferencetotheimaginaldiscsfromwhichtheaffectedstructuresderive.Foreachthoracicdisca“targetcenter”wascalculatedastheweightedmeanvalueofallbeamlocationsaffectingtherespectiveadultderivatives.Thetargetcentersforthegermbandstagemapwithintherespectivegermbandsegments.Thepatternoftargetcentersfortheblastodermstageiscomparabletothethoracicregionofpublishedfatemaps,andthedistancesbetweenadjacentlegcenters(approximatelythreecelldiameters)agreewithrecentevidencebasedonmosaicflies.Wediscussthequestionwhetherthetargetcentersmarkthepositionoftherespectivediscprogenitorcellsatthestagesofirradiationandconcludethatthesepositionsarerenderedrathercorrectlyatleastwithreferencetothelongitudinaleggaxis.

[学科分类] 生物科学（综合）