The identification of myogenic cells in regenerating skeletal muscle: I. Early anuran regenerates☆
[摘要] Muscleregenerationisusuallyconsideredtoinvolvetwoeasilyrecognizablemononuclearcellpopulations:(a)irregularlyshapedphagocyticmacrophages;and(b)fusiformmyogeniccellswhichresidebeneaththebasallaminaoftheinjuredmyofibers.Thepresentstudyfindsthatanuranmacrophagescanmimicearlymyogeniccellsbyadoptingafusiformshapeandasublaminarpositionduringtheinitialstagesofphagocyticinfiltration.ImplantsofmincedgastrocnemiusmusclefromadultRanapipiensandR.clamitanswereused.Macrophagecytologywasexaminedinnormallyregeneratingimplantsandimplantsmixedwithcarbontracerparticles.Implantsofnonlivingmuscle,lyophilizedorfrozenrepeatedlypriortoimplantation,werealsopreparedtostudytheprocessofmacrophageinvasionfreefromcontaminationbyanyendogenousmyogeniccells.Ultrastructuralexaminationofnormalimplantsfindsnoclearmorphologicalboundariesbywhichearlymyogeniccellsandmacrophagescanreliablybeseparated.Studiesofcarbon-markedimplantsdemonstratetheoccurrenceofnumerous,carbon-labeledfusiformsublaminarmacrophages.Fusiformmacrophagesarealsofoundinthemyofibersoftheimplantedmuscletissueskilledbypreviousfreezingorlyophilization.Theseobservationsindicatethatthecriteriaofcellshapeandlocationtraditionallyusedformyogeniccellidentificationareofdoubtfulvalidityandmustbereevaluated.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
