Regulation of ribosomal RNA synthesis and its bearing on the bobbed phenotype in Drosophila melanogaster
[摘要] TherateofribosomalRNA(rRNA)synthesiswasstudiedbothinvitroandinvivousingovariesfromDrosophilamelanogasterstockscarryingdifferentamountsofDNAcomplementarytoribosomalRNA(rDNA).ItwasshownthattherateofrRNAsynthesisisreducedinfliesexhibitingthebobbedphenotypeandhavinganrDNAcontentlowerthan0.130%.TheseflieshavebeenshowntolayeggsatareducedratebutofnormalRNAcontent.Ovariesfromphenotypicallywildflies,withrDNAcontentsof0.180%,0.370%,and0.580%showedinsteadaboutthesamerateofrRNAsynthesis.FemaleswithsuchrDNAcontentslayeggsofthesameRNAcontentandatthesamerate.ThesedataallowedustopostulatetheexistenceofregulatorymechanismsforrRNAsynthesis.Inspiteofthereducedrateofsynthesisincertaintissues,bobbedflies(bothmalesandfemales)havebeenshowntohavethesameRNA/DNAratioasphenotypicallywildflieswithdifferentrDNAcontents.Thesameresultshavebeenobtainedusinganintermediatestage(pupae).Itisknownthatbobbedfliesexhibitadelayeddevelopment.ProbablythisallowsforanormalizationoftherRNAcontent.Thegenesisofthebobbedphenotypeisdiscussedonthebasisoftheresultsobtained.
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[效力级别] [学科分类] 生物科学(综合)
[关键词] [时效性]