Control of phenotypic expression in cultured chondrocytes: Investigations on the mechanism☆
[摘要] Whengrowninasuitablemedium,chickchondrocytesretainthemorphologycharacteristicofcartilagetissueandsynthesizeamatrixwhosemainconstituentischondroitinsulfate.Ifchickembryoserumisaddedtotheculturemedium,thecellsattainafibroblastlikemorphologyandstopsynthesizingchondroitinsulfate.AstudyofthemetabolicfateofradioactiveglucosamineinwholecellshasrevealedablockatthelevelofUDP-NAG-pyrophosphorylasereactioninthefibroblastlikecultures.Thisblockcanexplainthecessationofchondroitinsulfateformation,sinceUDP-NAGisanimportantsubstrateinthepolymerizationofthisglycosaminoglycan.AconsiderablelevelofUDP-NAG-pyrophosphorylasewasdemonstratedinthehomogenatesofthefibroblasticcells.Variouspossibilitiesarediscussedconcerningthemechanismofthisblockanditsrelationtotheinhibitionofmatrixformation.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
