Polysomes and RNA synthesis during early development of the surf clam Spisula solidissima☆
[摘要] RNAandproteinsynthesisandpolysomeformationwerestudiedduringearlydevelopmentofSpisulaembryos.Theoocytehasasmallnumberofpolysomesandalowbutmeasurablerateofproteinsynthesis(leucine-3Hincorporation).Afterfertilization,thereisacontinualincreaseinthepercentageofribosomessedimentinginthepolysomeregion.NewlysynthesizedRNA(uridine-5-3Hincorporation)wasfoundinpolysomesasearlyasthe2-cellstage.Duringcleavage,thenewlyformedRNAisassociatedmainlywiththelightpolysomes.RNAextractedfrompolysomeslabeledatthe4-cellstageispolydisperse,nonribosomal,andnon-4S.ActinomycinDcausesareductionofabout30%ofthepolysomesformedbetweenfertilizationandthe16-cellstage.Intheearlycleavagestagesthelightpolysomesaremostlyaffectedbyactinomycin.
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[效力级别] [学科分类] 生物科学(综合)
[关键词] [时效性]