Two-dimensional gel patterns of protein synthesis before and after fertilization of sea urchin eggs☆
[摘要] EggsandembryosoftheseaurchinLytechinuspictuswerelabeledwith[35S]methionine.Aqueousextractsofproteinwerepreparedandanalyzedbyahighresolutiontwo-dimensionalpolyacrylamidegelelectrophoresissystemdescribedrecentlybyO'Farrellutilizingisoelectricfocusingandsodiumdodecylsulfateelectrophoresis.Outofabout400distinctlyresolvednewlysynthesizedproteins,allbutafewdetectableinthezygotewerebeingsynthesizedintheegg.Thus,theactivationoftranslationofstoredmaternalmessengerRNAfollowingfertilizationisduetoaquantitativeratherthanqualitativechangeinthepopulationofmessengerRNAavailablefortranslation.Thepatternsofproteinsynthesischangeonlyslightlyduringcleavage,butmajordifferencesappearbythebeginningofgastrulation.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
