A reexamination of the leucine tRNAs and the leucyl-tRNA synthetase in developing Tenebrio molitor
[摘要] ThetranslationalcontrolmechanismpreviouslyproposedforthesynthesisofadultcuticularproteinsinTenebriomolitorisdependentupontheappearanceofamajor,novelleucinetRNAandachangeinleucyl-tRNAsynthetaseactivityjustpriortoadultemergence.Thepropertiesoftheleucyl-tRNAsynthetaseextractedfrompupaewerereexamined.Underoptimalaminoacylationconditions,nonewleucineisoacceptingtRNAswereobservedduringdevelopment.However,undersuboptimalconditions,adifferentialchargingoftheleucinetRNAspecieswasnoted.Thechromatographicprofilesofleucyl-tRNAsaminoacylatedinvivoinbothearlyandlatepupaewerefoundtobethesameandwereidenticaltotheprofilesobtainedbychargingtRNAsinvitro.PreviousevidenceforatranslationalcontrolsystemoperatinginTenebrioisdiscussedinrelationtotheseresults.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
