Brain and ganglion development from two genotypic classes of cells in allophenic mice☆

[摘要] Severalstrain-specificmarkerswerefoundtobehistochemicallyvisualizableinpartsofthecentralnervoussysteminallophenicmice.Thesemarkersthereforeprovideanewbasisformappingthenormaldevelopmentallineagesofmajorpartsofthenervoussystem,andforidentifyingthefocusofmutantgeneactioninsomeneurologicalmutations.Cellstrainsinmosaicanimalswerevisualizedonthebasisofaquantitativedifferenceinβ-galactosidaseactivity(Bgs-locus),inthePurkinjezoneofthecerebellum,andinthehippocampalpyramidalzoneofthecerebrum.Thedifferentialbetweenstrainswasincreasedifthebeige(bgJbgJ)mutationwasincludedinthehigh-activitystrain.(β-galactosidaseislysosomal,andenhancedvisualizationinbeigeresultsfromitsenlargedandaggregatedlysosomes.)Purkinjecell-strainvisualizationwasalsoobtainedbyanindirectfluorescentantibodytechnique,insectionstreatedwithantiseracontainingantibodiesagainststrain-typehistocompatibilityalloantigens,includingH-2.Theabovemarkersrevealconsiderableinterspersionofcellsfromseparatelineagesinshortsequencesofeachgenotype.Purkinjeandpyramidalcellsofthesamebrainsometimesdifferappreciablyingenotypiccomposition.Theenzymeglucosephosphateisomerasewasfoundhistochemicallytobelocalizedinnervefibersratherthancellbodiesinthebrain.However,itwasprominentinthecellbodiesofthespinalganglia,sothatbiochemicaldeterminationofganglionstrain-typesispossiblebymeansofstrain-specificisozymes(Gpi-1-locus).Individualgangliacontainedbothcellstrainsandthusarenotindividuallyderivedasclonesfromtheneuralcrest.

[学科分类] 生物科学（综合）