Identification of hemoglobin types contained in single chicken erythrocytes by fluorescent antibody technique☆

[摘要] Ithasbeensuggestedthattheswitchinhemoglobin(Hb)types(fromembryonictoadult)duringchickenembryonicdevelopmentisassociatedwiththesubstitutionofoneerythroidcellline(“primitive”)foranother(“definitive”).ForthedetectionoftwoHbtypesinsidesingleerythroidcells,rabbitantibodiesspecificforembryonicandadultHbswereprepared.RabbitantibodyspecificforembryonicHbcross-reactedonlywithembryonicmajorHbcomponents,whileantibodyspecificforadultHbdidsolelywithadultminorHbcomponent.Theantibodieswereconjugatedwithfluoresceinisothiocyanate.Theconjugatedantibodieswereusedforthefluorescentstainingofbloodsmearsofdevelopingchickenembryosatdifferentages.DirectfluorescentantibodytechniquedemonstratedthatthemajorcomponentsofembryonicHbandtheminorcomponentofadultHbwerenotpresentwithinthesameerythrocyteduringchickenontogenesis.Itstronglysuggestedthatembryonic-typeHbandadult-typeHbdonotcoexistwithinthesamecell.

[学科分类] 生物科学（综合）