The possible differentiation of osteogenic elements in vitro from chick limb mesodermal cells: I. Morphological evidence☆
[摘要] Inthedevelopingchicklimbbud,myogenic,fibrogenic,chondrogenic,andosteogenictissuesarederivedfromembryonicmesenchyme.Previousreportsshowthatwhenstage24limbmesenchymalcellsareculturedinvitro,chondrocytes,fibrocytes,andmyocytescanbeidentifiedonthebasisofmorphologicalandbiochemicalparameters.Thestudiesreportedhereclearlydemonstratethat,insimilarcultures,crystallinecalciumphosphatematerialisdepositedinthechondrocyticandfibrocyticmatrices.SuchcrystallinematerialisnotobservedbeforeDay10ofculturelife.SubsequenttoDay10ofculture,firstamorphous,thencrystallinecalciumphosphateisobserved.Onthebasisoflightandelectronmicroscopicanalysis,itappearsthattheinvitrocalcificationphenomenoncloselyresemblesthemorphologicalandtemporalsequenceofosteogenesisobservedinvivo.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
