Defective development after puncturing the periplasm of nuclear multiplication stage Drosophila embryos☆
[摘要] Nuclearmultiplicationstageembryoswerepuncturedineithertheanterior,midlateral,orposteriorregions.Bothembryosandadultswereexaminedfordefects,andthedefectswerecorrelatedwithwhethertherehadbeenanyleakageofcytoplasmicmaterialfromtheeggatthetimeofpuncturing.Embryonicdefectswerefound,correlatedtothesiteofdamage,inallthreeregions.Anumberofembryoswasfollowedthroughdevelopmentanditwasfoundthat15.1%oftheembryoswhichleakedcytoplasmhatchedintolarvae,comparedto82.3%ofthosewhichdidnotleakanycytoplasm.Morphologicaldefectsarisingasaresultoflateralpunctureonlywereobservedinadults.Manysterileadultswereobtainedfromeggsinwhichtheposteriorregionhadbeenpunctured.Theresultsshowthatnuclearmultiplicationembryosarewellabletotoleratethedisturbanceofthecorticalcytoplasmcreatedbypuncture,butonlyrarelyaretheyabletocompensatefortheactuallossofmaterialbyregulation.TheresultsweresimilartothoseobservedafterpuncturingDrosophilaembryosatthecellularblastodermstage.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
