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δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells
[摘要] Increasingspecializationforδ-crystallinsynthesisisaprominentfeatureofthedifferentiationofchicklensepithelialcellsintolensfibercellsandcanbestudiedinculturedembryoniclensepithelia.Quantitationofδ-crystallinmRNAbymolecularhybridizatontoa[3H]DNAcomplementarytoδ-crystallinmRNAdemonstratesthatdifferentiation,bothinovoandintissueculture,isassociatedwiththeaccumulationofδ-crystallinmRNA.Inthecultures,thereisanoverallstimulationofproteinsynthesis,includingδ-crystallinmRNAduringthefirst5hrinvitro.Between5and24hrinvitrothereisadifferentialstimulationofδ-crystallinsynthesisandanaccumulationofδ-crystallinmRNAthatcanquantitativelyaccountforthisstimulation.
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[效力级别]  [学科分类] 生物科学(综合)
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