Organ cultures of the embryonic rat parietal yolk sac: II. Synthesis, accumulation, and turnover of collagen and noncollagen basement membrane glycoproteins☆

[摘要] Synthesis,accumulation,andturnoverofbasementmembranecomponentshavebeenstudiedinorganculturesof13.5-and14.5-dayembryonicratparietalyolksactissuesonanutrientagarsubstrate.Thebiochemicalstudiesdescribedinthisreportwerecorrelatedwithmorphologicandautoradiographicstudiesdescribedinthecompanionpaper(Minoretal.,Develop.Biol.48,1976).Thesestudiesshowedthatbasementmembraneistheonlyextracellularmatrixsynthesized,itisonlysynthesizedbytheparietalendodermalcells,anditssynthesisismaintainedforatleast6days.Inthesecultures,synthesisanddegradationofcollagenandnoncollagenproteinsvariedindependentlyinresponsetoenvironmentalchanges,suchasthefrequencyoffeedingorpresenceoftrophoblast.Theturnoverofbasementmembranecollagenwasmuchslowerthanthatofthenoncollagenproteinsandthisdifferenceintherateofturnoverofthecomponentshadamajorroleindeterminingthecompositionofthenewlysynthesizedbasementmembrane.

[学科分类] 生物科学（综合）