Denervation effects on aspartate carbamyl transferase, thymidine kinase, and uridine kinase activities in newt regenerates☆
[摘要] Activitiesofenzymesinthedenovopathway,aspartatecarbamyltransferase,andsalvagepathway,thymidineanduridinekinases,fornucleotideprecursorsynthesiswereexaminedunderconditionsofinnervationanddenervationintheamputatednewtforelimb.ItwasfoundthatACTaseanduridinekinasewereactivethroughoutregeneration(Days0–26)ofinnervatedlimbs,andthatthymidinekinasebecameactiveonlyatthenerveindependentstage.Denervationslightlyreducedthelevelsofactivitybutdidnotalterthepatternofactivityforanyoftheseenzymesforupto26daysaftersimultaneousamputationanddenervation.Reductioninlevelsofactivityindenervatedlimbsbecamemoreprominentastheinnervatedcontrollimbsenteredthenerveindependentstage,butatnotimethroughoutthe26-dayperioddidlevelsofactivityindenervatesfalltobaselinevalues.NervesapparentlyhavelittleeffectontheunderlyingmetabolicpathwayswhichproduceprecursorsforDNAsynthesis.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
