Isolation and characterization of mutations affecting UDPG pyrophosphorylase activity in Dictyostelium discoideum☆
[摘要] AprocedureforscreeninglargenumbersofclonesforanenzymeactivitywasusedtoisolatemutationswhichaffectUDPGpyrophosphorylaseactivity(EC2.7.7.9)inthecellularslimemoldDictyosteliumdiscoideum.FivestrainswererecoveredwhichhavelittleornoUDPGpyrophosphorylaseactivity.Tenotherstrainswerefoundwhichhavesignificantactivityinvivowhichisrapidlyinactivateduponcelllysis.ThesestrainshavepermittedustoevaluatetheroleofUDPGpyrophosphorylaseduringgrowthanddevelopment.Theenzymeaffectsthegrowthrateofthecellsbutisnotessentialforgrowth.However,duringdevelopmentthelackofenzymeactivityleadstocelldeathandlysis.StrainswhichlackUDPGpyrophosphorylaseaccomplishearlydevelopmentaleventsbutareunabletoculminate.However,certainbiochemicalandcytologicaldifferentiationsassociatedwithlatestageswereobserved.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
