Spermatozoan response to the toad egg matured after removal of germinal vesicle
[摘要] Thegerminalvesicle(GV)wasremovedfromtoadoocytesatvarioustimesaftertreatmentwithprogesterone,andenucleatedeggswereinseminatedunderconditionsthatensuredfertilizationofnucleatedcontroleggs.TheeggsenucleatedbeforetheinitiationofGVbreak-downdidnotshowgenuinecleavage.Cytologicalexaminationsrevealed,however,thatspermatozoaentertheeggsenucleatedevenbeforethehormonetreatment,withoutsubsequentformationofpronucleiorDNAsynthesis.Thesamelackofresponsewasobservedwhenseveraldetergent-pretreatedspermwereinjectedintoenucleatedeggs.WhenGVmaterialwasinjectedbackintoenucleatedoocytes,theinjectedspermatozoaunderwenttransformationandDNAsynthesis,althoughinvariabledegrees,intheeggcytoplasm.ItisconcludedthattheeggbecomesfertilizableindependentlyoftheGVduringthehormone-inducedmaturationprocess.Afterenteringtheegg,however,spermatozoarequireGVmaterialfortheirparticipationinthedevelopmentalprocess.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
