Trehalose accumulation in stalk and spore cells of Dictyostelium discoideum☆
[摘要] Single-celledmyxamoebaeundergodifferentiationintoeitherstalkcellsorsporecellsduringa24-hrperiodinDictyosteliumdiscoideum.Thisstudyemployedultramicrochemicaltechniquesandenzymaticcyclingtoassessthepresenceofcell-specificeventsinsporeandstalkcells.Freeze-driedsectionsofoneorganismwereassayedin0.1μlofreactionmixture.Thismethodwasusedtodeterminetheextentoflocalizationoftrehaloseinsporecellsandstalkcellsduringdevelopment.Trehalosewaslowintheearlystagesofdifferentiationtoabout20hrwhenthelevelstartedtoincrease.Indevelopingsporecells,thetrehaloselevelincreasedsixfoldduringthelast5hrofdevelopment.Likewise,theentirestalkcontainedtrehalosewhenthestalkwasfirstformed.Atmaturesorocarp,trehaloselevelswerethesameinsporesandtheapexofthestalk.Therewasadecreasinggradientoftrehalosedownthestalk.Thebottomone-fourthofthestalkwasdevoidofthisdisaccharide.Therefore,trehalosewasdegradedfromanareaofthestalkwhereitwaslocalizedearlierindevelopment.Theresultsofthisinvestigationnegatetheassumptionthattrehaloseisneverpresentinthestalk.Althoughtrehalosewasfoundinsporecells,prestalkcellsalsocontainedhightrehaloselevels.Thestalkcell-specifictrehalosewasnotretainedduringdifferentiation,however,butwasapparentlydegradedinthematurestalkcell.
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[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
