Viral transformation of rat myoblasts: Effects on fusion and surface properties☆
[摘要] Linesofratmyoblastsinfectedbyaviansarcomaviruseshavebeenisolated,cloned,andusedtostudytheeffectsofviraltransformationonmyogenicdifferentiationandthesurfacechangesassociatedwithdifferentiation.Thelinestransformedbysarcomavirusesfailedtofuseintomyotubesanddidnotshowtheincreaseinmyosinsynthesisnormallyassociatedwithfusion.Theparentalnontransformedlineshowed,subsequenttofusion,asurfacealterationdetectablebyexternallabelingmethods.Thisalteration,anincreaseinthelevelofanexternalproteinofMW>200×103,issimilartothatobservedinfibroblastsarrestedintheG1phaseofthecellcycle.Thisproteinwasabsentorgreatlyreducedonthesurfacesofthemyoblastlinesthathadbeentransformedbysarcomaviruses.Therefore,viraltransformationcauseslossofseveralpropertiesnormallyassociatedwitharrestofmyoblastsinG1.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
