The relation between villus formation and the pattern of extracellular fibers as seen by scanning microscopy☆
[摘要] Duodenalmorphogenesisinthechickembryohasbeenstudiedtoseeifchangesinorganizationofextracellularfibersareassociatedwithvillusformation.Threemajorprocessesoccurduringmorphogenesis;longitudinalridgesform,thentheseridgesbecomezigzaginshape,andfinallyadoublerowofvilliformfromeachzigzagridge.Tissuesofdifferentdevelopmentalstageswereprogressivelytrypsinizedtoremovecellularmaterialandwerepreparedforscanningmicroscopytoshowtheorganizationofextracellularfibers.Resultsshowthatfibersystemsofincreasingcomplexityformasthedudoenumdevelops,andsuggestthatsomecellulareventssuchasinitialridgeformationprecedethesechanges.Tissueswithlongitudinalridgescontainonlyrandomlyorganizedfibers.Intissueswithzigzagridges,orientedfibersappearalongtheridgesandsomeextendlaterallyfromflexuresofeachzigzagridge,butinterridgefibersarestillrandomlyorganized.Whenvilliform,fibersinthebodyofthevillusarerandombutfibersatthebaseofvilliandbetweenvilliarehighlyoriented.Transmissionmicroscopyandcollagenasetreatmentofpartiallytrypsinizedtissueindicatethatmost,ifnotallextracellularfibersviewedbyscanningmicroscopyarecollagenous.Therefore,sincecollagenfiberorganizationissocloselyrelatedtomorphogeneticchanges,wepresumeitplaysanimportantrole.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
