Cell interactions and the regulation of cholinergic enzymes during neural differentiation in vitro☆

[摘要] Seven-day-oldchickembryoneuralretina(NR),telencephalon(T),opticlobe(OL),andrembencephalon(Ro)weredissociated,andtheresultingcellsuspensionswereallowedtoreaggregateinvitroduring3dayseitherindependentlyorindifferentbinarycombinations.Interactionscouldbedetectedbythecomparisonoftheactivityoftheenzymesofthecholinergicsystem,cholineacetyltransferase(CAT)andacetylcholinesterase(ACE),in“pure”and“combined”aggregates.Theresultsclearlyshowthattheactivityofbothenzymesinembryonicneuralcellscanbemodifiedselectivelybyinteractionsbetweendifferentcellpopulations.Thus,combinedNR-OLaggregatesshowanincreaseinCATwithoutchangesinACE,NR-TanincreaseinCATandadecreaseinACE,T-RoadecreaseinbothCATandACE,andOL-Tnochangesatall.ExperimentsinwhichNRandOLcellswerecombinedindifferentproportionsindicatethattheinteractionsrequirethepresenceofdefinednumbersofcellsfromeachkind.Isochronousandheterochronouscombinationsof7-and10-day-oldNRandOLcellsshowthattheinteractivecapacitiesofthecellschangewithdevelopment.

[学科分类] 生物科学（综合）