Characterization and developmental regulation of β-galactosidase isozymes in Dictyostelium discoideum☆
[摘要] Twoisozymesofβ-galactosidase(EC3.2.1.23)havebeenidentifiedduringgrowthanddevelopmentofthecellularslimemold,Dictyosteliumdiscoideum.Theisozymeshavebeenpartiallypurifiedanddifferinavarietyofphysicalandenzymaticproperties.β-Galactosidase-1ispresentinvegetativecells.Thespecificactivityisreducedduringearlydevelopmentandthenincreasesagainduringculmination.Thespecificactivityofβ-galactosidase-2increasesinearlydevelopmentandthenagainduringculminationandsporematuration.Thespecificactivityofβ-galactosidase-2isextremelydependentupongrowthconditionsandisregulatedovera160-foldrange.TheaccumulationofbothisozymesisdependentonconcomitantRNAandproteinsynthesis.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
