Physiological and structural properties of colchicine-treated chick skeletal muscle cells grown in tissue culture

[摘要] Chickmusclecellsgrownintissue-culturemediumcontainingcolchicinedevelopedintoroundedcellscalled“myosacs.”Electronmicrographsofmyosacsweresimilartountreatedmyofibersexceptthatmicrotubuleswereabsentandthecontractilematerialwasdisorganized.Mostoftheelectrophysiologicalcharacteristicsofmyosacsweresimilartothoseofuntreatedmyofibers.Thus,bothcellulartypeshadsimilarrestingpotentials,nonlinearcurrentvoltagecurves,threetypesofactionpotentials(Na+,Ca2+,andCl−spikes),andacetylcholinesensitivitywith“hotspots.”Bothdemonstratedcontractionwithelectricalorchemical(acetylcholine,caffeine)stimuli.Theonesignificantdifferencewasthatmyosacs,unlikemyofibers,werealwaysisopotentialthroughouttheirintracellularspace.

[学科分类] 生物科学（综合）