Isolation and localization by histoimmunology of isoperoxidases specific for male flowers of the dioecious species Mercurialis annua L.
[摘要] SpecificanodicisoperoxidasesofmaleflowersofthedioeciousplantMercurialisannuawereextracted,partiallypurified,andinjectedintorabbits.Theserumagainstthesespecificperoxidaseswasusedafterabsorptiontolocalizethesemaleisoenzymesduringflowerdevelopmentbymeansofhistoimmunology.(Indirectimmunoperoxidasemethod).Theperoxidaseswerecharacteristicofmicrosporogenesisandtapetumdifferentiation.Theirsynthesisstartedatanearlystageofmaleorganogenesis.Theywerenotobservedinothersitesofperoxidaseactivityofmaleflowers(tunica,endothecium,filament,vascularanatomy)orinfemaleflowers(embryosacornucellus).Iproposethattheseisoenzymesconstituteanearlyandspecificmarkerofmaleorganogenesisinhigherplants.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
