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Follicle cell protein synthesis and its contribution to the yolk of the Cecropia moth oocyte
[摘要] TheworkpresentedinthisreportdescribesthepatternofpolypetidessynthesizedbythefollicularepitheliumfromthevitellogenicCecropiamothfollicle.Theroleofthefolliclecellproductineggformationisalsodiscussed.Theexperimentalapproachinvolved:(1)incubationoflivingmaterialwithalabeledprecursor;(2)isolationofspecificpartsofthefollicle;(3)solubilizationofthesampleusingsodiumdodecylsulfateanddithioerythritol;(4)identificationofthelabeledpolypeptidesbypolyacrylamidegelelectrophoresis.Theresultsshowthatthefollicularepitheliumfromvitellogenicfollicleslabelsawidevarietyofelectrophoreticallydifferentpolypeptides.Itisalsodemonstratedthatonelabeledproductissecretedbythefollicularepithelium,takenupbytheoocytes,andthenpackagedintotheyolkspheres.
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[效力级别]  [学科分类] 生物科学(综合)
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