Follicle cell protein synthesis and its contribution to the yolk of the Cecropia moth oocyte
[摘要] TheworkpresentedinthisreportdescribesthepatternofpolypetidessynthesizedbythefollicularepitheliumfromthevitellogenicCecropiamothfollicle.Theroleofthefolliclecellproductineggformationisalsodiscussed.Theexperimentalapproachinvolved:(1)incubationoflivingmaterialwithalabeledprecursor;(2)isolationofspecificpartsofthefollicle;(3)solubilizationofthesampleusingsodiumdodecylsulfateanddithioerythritol;(4)identificationofthelabeledpolypeptidesbypolyacrylamidegelelectrophoresis.Theresultsshowthatthefollicularepitheliumfromvitellogenicfollicleslabelsawidevarietyofelectrophoreticallydifferentpolypeptides.Itisalsodemonstratedthatonelabeledproductissecretedbythefollicularepithelium,takenupbytheoocytes,andthenpackagedintotheyolkspheres.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学(综合)
[关键词] [时效性]