Undersulfated chondroitin sulfate in the cartilage matrix of brachymorphic mice
[摘要] Homozygousbrachymorphic(bmbm)micehaveadisproportionatelyshortstature,similartohumanachondroplasia.Wepreviouslyshowedthateachzoneofgrowthinyoungbmbmepiphysealcartilagesissmallerthannormalandthattheextracellularmatrixappearstocontainnormalcollagenfibrils,butsmallerandreducednumbersofproteoglycanmatrixgranules.Ourstudiesreportedhereindicatethatmutant,likenormalcartilage,synthesizestypeIIcollagenandcontainsnormalquantitiesofglycosaminoglycansasjudgedbyuronicacidcontent.However,theglycosaminoglycansfromthemutantdifferfromthenormalintheirchromatographicandelectrophoreticproperties.Furtherstudiesestablishedthatglycosaminoglycansfromcartilagesofbrachymorphicanimalswereundersulfated.WhereaschondroitinasedigestsofglycosaminoglycansfromcartilageofnormalC57Bl6J5-day-oldmicecontainedpredominantlydisaccharidessulfatedinthe4-position,thatofthemutantcontainedappreciableunsulfateddisaccharidesaswell.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
