In vitro stimulation of cartilage in embryonic chick neural crest cells by products of retinal pigmented epithelium☆

[摘要] Theretinalpigmentedepitheliumofthechickembryoinfluencesheadneuralcrestmesenchymalcellstoformthescleralcartilageoftheeye.Thepossibleroleofextracellularmatrixinthisinteractionwasstudied.ExtracellularmatrixwasdepositedonMilliporefiltersinvitrobypigmentedepithelialcellswhichwerethenkilledbydistilledwaterlysis.WhengrownontheMilliporefilterswhichhadcarriedpigmentedepithelium,clonalneuralcrestandperiocularmesenchyme“target”cellsformedcartilagein61of155experiments.CartilagewasnotformedwhenthecellsweregrownonnakedfiltersnordidgelsofpurifiedTypeIandTypeIIcollagenpromotechondrogenesis.Itisconcludedthatextracellularmatrixdepositedbythepigmentedepitheliuminvitroisapotentstimulusfortheinductionofchondrogenesisincompetentmesenchyme,andthatlivingpigmentedepithelialcellsneednotbepresentforsuchinduction.

[学科分类] 生物科学（综合）