Protein synthesis and messenger RNA stability in activated, enucleate sea urchin eggs are not affected by actinomycin D☆
[摘要] WehaveinvestigatedthepossibleeffectsofactinomycinonmRNAhalflifeandonproteinsynthesisinactivated,enucleateseaurchinhalf-eggs.Therewasnoeffectby20μg/mlactinomycinDontheoveralllevelofproteinsynthesisforupto12hr.Actinomycin-treatedparthenogeneticmerogonesproducedthesamespectrumofproteinsasuntreatedmerogones,asdeterminedbydoublelabeldiscgelelectrophoresis.Actinomycinhadnoeffectonthelevelsofpoly(A)-containingRNAinmerogonesculturedforupto10hrinthepresenceoftheinhibitor.WeconcludethatinthissystemactinomycinhasnogrosseffectuponproteinsynthesisnoruponthestabilityofmRNAanddoesnotinducedifferentialdecayofspecificmRNAspecies.Thus,experimentsconductedusingactinomycintoprobeproteinsynthesisintheabsenceofnucleartranscriptioninseaurchinembryosarevalid.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
