Promotion of retinal neurite outgrowth by substratum-bound fibronectin☆
[摘要] Wehaveexaminedconditionsunderwhichaggregatesofembryonicchickneuralretinawillextendneuritiesinvitro.Trypsin-dispersedcellsfrom7-dayembryonicchickneuralretinawereaggregatedinrotationculturefor8hrandmaintainedinserum-freemediumonavarietyofstandardculturesubstrate.Aggregatesextendfewneuritesonuntreatedplastic,glass,orcollagensubstrata.However,pretreatmentofthesesubstratawithhumanplasmafibronectinenhancestheircapacitytosupportretinalneuriteoutgrowth.Aggregatesculturedonfibronectin-treatedsubstrataextendlong,radiallyorientedneuriteswithin36hrinvitro.Themorphologyoftheseneuritesisdistinctfromthatseenwhenaggregatesareculturedonpolylysine-treatedsubstrata.Inthelattercase,neuritesarehighlybranchedandgrowconcentricallyaroundtheaggregateperimeter.Additionoffibronectintopolylysine-treatedsubstratastimulatesradialneuriteoutgrowth.PromotionofneuriteoutgrowthisdependentontheamountoffibronectinboundtotheculturesubstratumandonthepHatwhichbindingoccurs.Therequirementsforfibronectin-mediatedneuriteoutgrowtharemorestringentthanthosepreviouslyreportedforfibroblastattachmentandspreading.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
