Enzymatic dissection of embryonic cell adhesive mechanisms: II. Developmental regulation of an endogenous adhesive system in the chick neural retina
[摘要] Previousstudieshavedemonstratedthepresenceoftwofunctionallydistinctintercellularadhesivesystemsoperatingamongembryonicchickneuralretinacells.Thesesystemsdifferintheirproteolyticsensitivity,protectionbycalciumagainstproteolysis,dependenceoncalciumforfunction,andinvitromorphogeneticpotential.Inthisreportwedemonstratethatfunctionalexpressionofthecalcium-dependentadhesivesystemofembryonicchickneuralretinacellsisdevelopmentallyregulatedbetweenDays7and16ofdevelopment,whereasthecalcium-independentadhesivesystemisnot.Age-dependentchangesaredescribedintermsoftheabilitytoproduceadhesive-competentcellsbearingthecalcium-dependentadhesivesystemandintermsoftheresponsesofthesecellsduringaggregationtoperturbationswithvariousdrugs.Enzymeandioncombinationsotherthancalciumandtypsinareshowntoyieldcalcium-dependentadhesive-competentcells.Wealsodescribetheprotectiveeffectofcalciumonthehistologicalandultrastructuralorganizationoftrypsinizedembryonicneuralretinatissue.Thepossibleroleofthecalcium-dependentadhesivesysteminretinaldevelopmentisdiscussed.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
