Translational control in sea urchin eggs and embryos: Initiation is rate limiting in blastula stage embryos☆
[摘要] Todeterminewhetherinitiationisrate-limitinginproteinsynthesisduringtheembryogenesisofseaurchins,polyribosomeprofilesofunfertilizedeggsandcleavage,blastulaandprismstageembryoswereexaminedafterincubationoftheeggsandembryosinthepresenceandabsenceoflowamountsofemetine,aninhibitorofpolypeptideelongation.Theribosomeswereradioactivelylabeledwith[3H]uridinebyinjectionoftheadultsduringoogenesissothatwecouldmonitoremetine-dependentshiftsofmonoribosomestopolyribosomes.Althoughinitiationisnotratelimitinginunfertilizedeggsor2-to16-cellembryosofStrongylocentrotuspurpuratus,itisratelimitinginblastulaandprismembryos.WesuggestthatinitiationbecomesratelimitingtoallowtheselectivetranslationofcertainclassesofmRNAduringlaterdevelopment.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
