Differential expression of aortic and lung elastin genes during chick embryogenesis☆
[摘要] Theratiosoftropoelastinbtoaweremeasuredinchickaortaandlungduringembryogenesis.Theratesoftropoelastinaandbsynthesisweredeterminedinshort-termorganculture.Theresultsdemonstratedthatinlungtissuetheratioofthetwotropoelastinsremainedessentiallyconstant.Eachofthetropoelastinscomprised50%ofthetotalelastinsynthesis.Intheaortictissue,tropoelastinbrepresented70%ofthetotalelastininthe11-to13-dayembryosandincreasedto91%byDay16.TheseobservationsseenintheorganculturesystemwereparalleledinmeasurementsoffunctionalmRNAscodingforthetwoproteins.MeasurementsoffunctionaltropoelastinmRNAsfrombothlungandaortictissueswereperformedinamRNA-dependentrabbitreticulocytelysatesystem.AlthoughthechangesintheabundanceofthetropoelastinmRNAsrevealedthesametrendasthatseenintheorganculturedata,themagnitudeofthetropoelastinbtoaratiointheaorticorganculturewastwicethatdeterminedinthecell-freetranslationofaorticmRNAs.Thedataobtainedfrombothcell-freetranslationsandorgancultureexperimentsdemonstratethatthereisadifferentialexpressionofelastingenesduringaortadevelopmentwhichissignificantlydifferentfromthatfoundindevelopinglung.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
