Expression of phenotypes in a temperature-sensitive allele of the apterous mutation in Drosophila melanogaster☆

[摘要] Atemperature-sensitivealleleoftheapterous(ap)locusofDrosophilamelanogasterhasbeenusedtoexaminethephenotypesproducedbythismutation,whichincludewing,mesonotal,andhalteredeficiencies,precociousadultdeath,andnonvitellogenicoocytedevelopment.Whenraisedat15°C,homozygousapts78jadultshavenearlywild-typewingmorphologyexceptforpatchesofmissingtriple-rowbristlesandposteriorwingmargindeficiencies.Withtheexceptionoftwomissingbristles,thedorsalmesonotumandthehaltereappearaswild-type.Increasingdeficiencyofstructuresderivedfromthewingandhaltereimaginaldiscsresultsfromincreasingculturetemperature,andat29°C,thewingblade,manydorsalmesonotalbristles,andthehaltereareabsent.Thetemperature-sensitiveperiodindevelopmentforthesedeficientphenotypesextendsfromlate-secondtomid-thirdinstar.Despiteextensivedeficienciesseenafterapts78jlarvaeareheat-pulsedat29°C,noduplicationofthenotalstructuresisevident,acommonresponseofothermutantshavingextensivewingdeficiencies.Whenraisedat29or25°C,apts78jadultsareshort-lived,andfemalesshownonvitellogenicoocytedevelopment.At22°C,however,adultsarelong-lived,andfemalesarevitellogenicandlayfertileeggs.Asharptemperature-sensitiveperiodforbothphenotypesislocatedduringthefirst24hrofpupaldevelopment.Theapplicationofajuvenilehormonemimic,ZR-515,restoredvitellogenesistoapts78jfemalesraisedat25°Cbutwasunabletorescuethemfromprecociousdeath.

[学科分类] 生物科学（综合）