Ontogeny and localization of the α, β, and γ crystallins in newt eye lens development☆
[摘要] Theα-,β-,andγ-crystallins,proteinscharacteristicforthevertebrateeyelens,havebeenlocalizedinthedevelopinglensofNotophthalmusviridescens,theeasternspottednewt.Usingtheimmunofluorescencetechnique,antibodiestotheα-,β-,andγ-crystallinclasseswereappliedtotissuesectionsthroughtheeyeregionofdevelopingN.viridescensembryos,Harrison(external)Stages30to46+.β-Crystallinswerethefirstofthecrystallinstoappearinafewcellsofthelensvesicleevenbeforethelengtheningoftheprospectiveprimaryfibercells.γ-Crystallinswerefirstdetectableataslightlymoreadvancedstageintheprospectiveprimaryfibers,andα-crystallinsinafewcellsofthebeginningprimaryfiberarea.Theexternallayer/epitheliumwasnegativeforβ-crystallinsuntillateinlensmorphogenesis,andα-andγ-crystallinscouldnotbedetectedinthesecellsatanytime.This,thefirstuseinamphibiaofhomologousantibodiesspecificforthecrystallinclasses,makesclearthatphylogeneticdifferencesexistastotheprimacyandrelevanceofspecificcrystallinstoeventsduringmorphogenesisoftheeyelens.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
