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A hydrogen peroxide block to polyspermy in the sea urchin Arbacia punctulata
[摘要] Fertilizationbymorethanonesperminseaurchinsinevitablyleadstounevendivisionanddeathoftheembryo.Weprovideevidenceforablockagainstthispolyspermyinvolvingthehydrogenperoxidereleasebytheeggduringfertilizationthatistriggeredbyentryofthesuccessfulsperm.Polyspermyin100%offertilizedeggswasdemonstratedwhencatalasewasaddedtodestroyhydrogenperoxideimmediatelyafterfertilization.Soybeantrypsininhibitor,anotherpolyspermicagent,isshowntopreventtheformationofhydrogenperoxideinthefertilizedegg.Thissuggeststhattheproteasereleasedfromeggcorticalgranulesduringfertilizationplaysaroleinthehydrogenperoxidegeneratingsystem.
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[效力级别]  [学科分类] 生物科学(综合)
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