Changes in the distribution of type IV collagen, laminin, proteoglycan, and fibronectin during mouse tooth development
[摘要] Thedistributionofcertainbasementmembrane(BM)componentsincludingtypeIVcollagen,laminin,BMproteoglycan,andfibronectinwasstudiedindevelopingmousemolarteeth,usingantibodiesorantiseraspecificforthesesubstancesinindirectimmunofluorescence.Attheonsetofcuspalmorphogenesis,typeIVcollagen,laminin,andBMproteoglycanwerefoundtobepresentthroughoutthebasementmembranesofthetooth.Fibronectinwasabundantundertheinnerenamelepitheliumattheregionofdifferentiatingodontoblastsandalsointhemesenchymaltissues.Afterthefirstlayerofpredentinhadbeensecretedbytheodontoblastsattheepithelial-mesenchymalinterface,lamininremainedincloseassociationwiththeepithelialcellswhereastypeIVcollagen,BMproteoglycan,andfibronectinweredistributeduniformlythroughoutthisarea.Laterwhendentinhadbeenproducedandtheepithelialcellshaddifferentiatedintoameloblasts,basementmembranecomponentsdisappearedfromthecuspalarea.ThesematrixcomponentswerenotdetectedindentinwhileBMproteoglycanandfibronectinwerepresentinpredentin.Theobservedchangesinthecollagenousandnoncollagenousglycoproteinsandtheproteoglycanappeartobecloselyassociatedwithcelldifferentiationandmatrixsecretioninthedevelopingtooth.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
