The effect of cytochalasin B and D on the fertilization of sea urchins☆
[摘要] Twoelectricaleventsoccuracrosstheplasmamembraneoftheseaurchineggduringtheinteractionwiththefertilizingspermatozoon;thefirst,astep-likedepolarizationpecedesthefertilizationpotential(FP)by11secat20°C.CytochalasinsBandDaltertheseeventsinseveralwaysdependingupondoseandtimeofexposureofthegametes.Whenboththeeggandthespermatozoonareexposedtothesemicrofilament-activedrugs,theformerforminuteswhilstthelatterintheorderofseconds,thedelaybetweenthetwoelectricaleventsisincreasedtwofold.Wesuggestthatamicrofilament-involvedprocessisoccurringduringthisdelayperiodwhich,althoughitselfnotalteringtheelectricalpropertiesoftheplasmamembrane,determinesthetimeofinitiationoftheFP.PretreatingspermatozoawithhigherdosesofeitherCBorCD,inhibitsfertilizationcompletelyorlowersthesuccessfulcollisionrate(α);wesuggestthatthetwodrugslowerαbyaffectingtheacrosomalregionofthespermatozoon.AlthoughbothdrugsincreasethedelayperiodtothesamedegreeCDhasfewersecondaryeffectsthanCB.
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[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
